These imagers or documentations have already been common in laboratory experiments especially in the field of molecular biology for a variety of uses. However, these are mainly used for the documentation of proteins and nucleic acids that were suspended in agarose or polyacrylamide gels. The SYBR Green is known to have stained this. In case you have no idea what this is made of, it has ultraviolet light transilluminators, hood, and protection from exposure of ultraviolet rays.
Bioolympics, Syngene, Biorad, and UVP were known to have been the primary manufacturers of these. These have grown to become more advanced as we know that technology keeps on improving anyway. Features have improved like handling chemiluminescence or fluorescence. For this segment, we shall find out tips on applying gel imaging systems to electrophoresis which is a common term established in similar experiments.
One of the struggles most lab professionals face is that they were not able to get the best resolution. Therefore, the results are not clear enough which is a bad thing to experience especially when investing on something like this is quite expensive. A tip is to vortex the protein samples both before and after processing the heating procedure. You can expect a great outcome after.
During the heating method, try not to boil those samples around one hundred degrees Celsius. The molecular weight will only get high whenever boiling it happens to be of really high temperature. Avoid those circumstances then and heat it up around seventy degrees Celsius instead. Just note that the preparation has a different degree too.
Another common mistake made by individuals is by not purchasing its other needed components. Some additional components may be necessary to enhance results. One of those is the 10 of 1x sample buffers. Applying this to well lanes that are unused is necessary. This lets the edge effects of gel to be avoided.
Speaking of buffers, there are some types that are disposed but others can be reused. That saves your budget for sure. Consider a buffer called the SDS PAGE running instead for it is reusable for two times and no background signals which are high are present. Just know that more than two can be done for reusing but there shall be an increase in its running time.
The bromophenol blue is something for us to consider by the way for it is of great importance. Not adding enough of it could become a major problem for sure especially in terms of its final resolution. When it gets loaded unto the wells, samples are definitely going to become difficult for us to see.
In electrophoresis, what you must know is that only a voltage which is constant can give constant mobility for proteins. Therefore, the constant power condition is not what is being referred to here. You better clear things out first.
Never ever let damages to occur because this protocol is significant. Each well should be inspected in case damage exists. Certain repairs and replacements might be necessary whenever you do find damages and other related problems.